Often yes, especially for international applicants whose primary language is not English, but waivers/exemptions can apply based on your prior schooling. Tufts Undergraduate Admissions states that applicants whose primary language is not English must submit proof of English proficiency unless they meet an exemption (such as sustained study in an English-instruction environment), and Tufts lists accepted tests like IELTS, TOEFL, PTE, and Duolingo (with some “indicator/home edition” restrictions depending on the test/version).
At the graduate level, requirements vary by school. For example, The Fletcher School publishes minimum acceptable TOEFL/IELTS scores in its admissions FAQ, and Tufts’ Graduate School of Arts & Sciences advises minimum recommended scores for TOEFL/IELTS/Duolingo for non-native English speakers.
From a Tufts recipient story perspective, Dalila Sumani shared, “I didn’t take any standardized tests,” highlighting that some applicants focus their strength in other parts of the application (though English testing requirements, when applicable, are separate from GRE/GMAT-style standardized tests).